Journal: Pediatric surgery international

Article Title: Transglutaminases, involucrin, and loricrin as markers of epidermal differentiation in skin substitutes derived from human sweat gland cells.

doi: 10.1007/s00383-009-2517-5

Figure Lengend Snippet: Fig. 3 a Epidermal substitute derived from SG cells. Stratum spinosum and granulosum (between arrows), TG1 positive (green), fluorescence microscopy 940. b Epidermal substitute derived from SG cells. Stratum basale, spinosum, granulosum, and corneum (between arrows), TG3 positive (green), fluorescence microscopy 940. c Epidermal substitute derived from SG cells. Stratum basale, spinosum, and granulosum (between arrows), TG5 positive (green), fluorescence microscopy 940. d Epidermal substitute derived from SG cells. Stratum spinosum, granulosum, and corneum (between arrows), involucrin positive (red), fluorescence microscopy 940

Article Snippet: Antibodies For immunofluorescence, the following antibodies were used: From LabVision (P.H.Stehelin&CIE AG, Basel, Switzerland): Involucrin (clone SY5, 1:100); from Abcam (Cambridge, UK): Loricrin (polyclonal, 1:500); from Novus Biologicals (Littleton, CO, USA): transglutaminase 1 (polyclonal, 1:800), transglutaminase 3 (polyclonal, 1:50), transglutaminase 5 (polyclonal, 1:200).

Techniques: Derivative Assay, Microscopy

Journal: Pediatric surgery international

Article Title: Transglutaminases, involucrin, and loricrin as markers of epidermal differentiation in skin substitutes derived from human sweat gland cells.

doi: 10.1007/s00383-009-2517-5

Figure Lengend Snippet: Fig. 2 a Normal human skin. Stratum spinosum and granulosum (between arrows), TG1 positive (green), confocal microscopy 940. b Normal human skin. Stratum granulosum and corneum (between arrows), TG3 positive (green), confocal microscopy 940. The remaining areas with seemingly positive signals are background signals. c Normal human skin. Stratum basale, spinosum, and granulosum (between arrows), TG5 positive (green), confocal microscopy 940. d Normal human skin. Stratum granulosum and corneum (between arrows), involucrin positive (red), fluorescence microscopy 940

Article Snippet: Antibodies For immunofluorescence, the following antibodies were used: From LabVision (P.H.Stehelin&CIE AG, Basel, Switzerland): Involucrin (clone SY5, 1:100); from Abcam (Cambridge, UK): Loricrin (polyclonal, 1:500); from Novus Biologicals (Littleton, CO, USA): transglutaminase 1 (polyclonal, 1:800), transglutaminase 3 (polyclonal, 1:50), transglutaminase 5 (polyclonal, 1:200).

Techniques: Confocal Microscopy, Microscopy

Journal: Redox Biology

Article Title: Neutrophil extracellular traps exacerbate inflammatory arthritis by inhibiting γδ Treg cell differentiation via the AIM2 inflammasome

doi: 10.1016/j.redox.2025.103881

Figure Lengend Snippet: NETs inhibited γδ Treg cell differentiation via their DNA components A-B. Pitstop 2, a CIE inhibitor (CI), was used to inhibit the endocytosis of NETs by γδ T cells. γδ T cells were pretreated with or without Pitstop 2 and subsequently stimulated with NETs. The levels of the NET components H3cit and MPO were assessed in γδT cells by immunofluorescence (A) and Western blot analysis (B). Scale bar = 5 μm. C. Internalization of SYTO Green-labeled NETs by γδ T cells was visualized by confocal microscopy. Cells were pretreated with or without Pitstop 2 prior to stimulation with labeled NETs. Scale bar = 5 μm. D. γδ T cells were pretreated with or without Pitstop 2 and subsequently stimulated with NETs. The proportion of γδ Treg cells among the total γδ T cells was analyzed via flow cytometry (n = 6). E. γδ T cells were stimulated with or without NETs or DNAse-treated NETs, and the proportion of γδ Treg cells among the total γδ T cells was analyzed via flow cytometry (n = 6). F. Relative protein levels of Foxp3 and p-STAT5 in γδ T cells stimulated with or without NETs or DNAse-treated NETs (n = 6). G. γδ T cells were cultured with or without a series of NET-associated DNA concentrations, and the proportion of γδ Treg cells among the total γδ T cells was evaluated by flow cytometry (n = 6). The data are presented as the means ± SEMs. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ns not significant, p > 0.05.

Article Snippet: For the indicated experiments, NETs, NET-associated DNA, a CIE inhibitor (Selleck, #S9670), an AIM2 inhibitor (MCE, #HY-144226), an AIM2 activator (Poly(dA:dT); MCE, #HY-138646), N-Acetyl- l -cysteine (1 mM, MCE, #HY-B0215), Tempo (10 nM, MCE, #HY-W001187) and Disulfiram (MCE, # HY-B0240) were added.

Techniques: Cell Differentiation, Immunofluorescence, Western Blot, Labeling, Confocal Microscopy, Flow Cytometry, Cell Culture

Journal: Cell reports

Article Title: Gene silencing by EZH2 suppresses TGF-β activity within the decidua to avert pregnancy-adverse wound healing at the maternal-fetal interface

doi: 10.1016/j.celrep.2022.110329

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Nikon Eclipse Ci-L microscope , Nikon , https://www.microscope.healthcare.nikon.com/products/upright-microscopes/eclipse-ci-series.

Techniques: Recombinant, Lysis, RNA Sequencing Assay, Expressing, Plasmid Preparation, Software, Microscopy